Document Type : Original Article

Authors

• Hosain Tavallali ¹
• mastaneh ostovar ²
• Mohamad Ali Karimi ¹
• Abolfath Parhami ³

¹ Full Professor, Department of Chemistry, Payam Noor University, Tehran, Iran

² Doctoral student of Payam Noor University, Tehran, Iran

³ Assistant Professor, Department of Chemistry, Payam Noor University, Tehran, Iran

Abstract

In the last few decades, a lot of research has been done based on various analytical methods in the measurement of amino acids in environmental and biological samples such as blood serum due to their important role in the functioning of the body's vital organs and also its indirect effect on the defense power. The use of colorimetric chemical sensors has attracted the attention of many researchers due to the simplicity of the method, good selectivity, cheapness, low equipment, no need for pre-preparation of the sample, usable for all samples, especially biological samples and detection with the naked eye. In this research, the colorimetric method based on ArsenazoIII (2,7-bis[(2-arsonophenyl) azo]-1,8-dihydroxy-3,6-naphthalenedisulfonic acid) (AAIII) (as a colorimetric chemical sensor) was used to determine arginine in aqueous medium. The addition of arginine to AAIII solution caused a visible color change from red to purple and a decrease in absorption at 536 nm wavelength. An isosbestic point was observed at 580 nm, which indicates the interaction between AAIII and arginine. The linear dynamic range for the UV-Vis absorption test was 15-340 μmolL-1. The detection limit was calculated to be 0.04 μmolL-1. This method was used to determine arginine in environmental, which had satisfactory resuls.

Keywords

• Chemosensor
• Colorimetry
• ArsenazoIII
• Arginine