Document Type : Original Article
Authors
1 Professor, Department of Chemistry, Payame Noor University, Tehran, Iran
2 Ph.D. Student, Payame Noor University, Tehran, Iran.
3 Assistant Professor, Department of Chemistry, Payame Noor University, Tehran, Iran.
Abstract
In this research, the design and development of Neutral Red (NHR), a commercial dye that is available with high purity, is reported as a colorimetric biosensor for the sequential identification of two amino acids, arginine (Arg) and glutathione (GSH). This method was used without the need for complex and expensive equipment and without using enzymatic methods to determine these two amino acids.
With the gradual increase of arginine, the absorption spectrum of the receiver decreased at 529 nm and increased at 454 nm, and the color of the solution changed from red to yellow. Further, by adding glutathione to the neutral red-arginine (NRH-Arg) complex, an increase in absorption at 529 nm and a decrease in absorption at 454 nm were observed, and the color of the solution changed from yellow to red.
In this study, the linear range for arginine and glutathione is 1.20-91.61 µmol/L and 243.11-605.49 µmol/L, respectively, and their correlation coefficient is 0.9945-0.9942, respectively. Also, the detection limit of the method is 0.61 and 25.32 µmol/L and the relative deviation for arginine at two concentrations of 0.10 and 0.50 µmol/L, respectively, and for glutathione at two concentrations of 0.370 and 0.98% and 1.17% respectively. 0.564 µmol/L was obtained, 1.20% and 1.08%, respectively. Finally, this method successfully identified and measured arginine in the blood serum samples of military barracks soldiers..
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