Document Type : Original Article
Authors
1 Department of chemistry payame noor university Tehran Iran
2 Department of chemistry payam noor University Tehran Iran
3 Department of chemistry payame noor University Tehran Iran
Abstract
Tetracyclines are a group of antibiotics widely known for their use in treating bacterial infections in both animals and humans. Therefore, accurate and rapid measurement of tetracycline levels is crucial for ensuring the safety of materials. This laboratory study presents an electrochemical aptasensor method for measuring tetracycline, which is based on the formation of a nucleic acid aptamer complex for detecting tetracycline on the electrode surface. This aptamer structure enhances sensitivity and accuracy in tetracycline measurement and it is prepared using cyclic voltammetry and square wave electrochemical techniques. The developed aptasensor in this study can potentially be used for detecting tetracycline in pharmaceutical preparations, contaminated food products, and drinking water. In addition to medical and food applications, this optosensor can be used as a potential tool for identifying chemical and biological agents in defense and military environments. The concentrations of tetracycline used in this experiment range from 10 µM to 10 mM, and they are analyzed using CV, impedance, and DPV graphs within a voltage range of -0.6 V to 0.6 V, a scan rate of 20 mV/s, a step potential of 2 mV, and a current range of 1.6 × 10⁻⁶ to 0 A. The results indicate significant improvement in detection limits and linear range. Additionally, this sensor features high sensitivity, stability, and reusability. This study presents significant improvements in tetracycline measurement and provides high efficiency in various applications of the drug.
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